Anti-Mena; clone A351F7D9

Analysis Note

ControlRat hippocampus tissue lysate

Application

Detect Mena using this Anti-Mena Antibody, clone A351F7D9 validated for use in WB, IH.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH, (Kwiatkowski, 2007).

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC, (Dent, 2007).

Research Sub CategoryCytoskeletal SignalingPI3K, Akt, & mTOR Signaling

Research CategoryCell StructureSignaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mammalian Enabled (Mena) is a protein belonging to ENA/VASP family, a group of proteins that are actin-associated and implicated in a variety cell processes. Specifically, Mena is involved in cell polarity, cell motility, cytoskeleton remodeling, filopodial, and lamellipodial dynamics within migrating cells. Three forms of Mena are expressed in embryonic head tissues and are identified based on molecular weight (140 kDa, 88 kDa, and 80 kDa). In adult wild-type tissues, Mena has been isolated in brain, testis, ovary, and fat in the 80 kDa form. Studies show the 140 kDa form is expressed only in adult brains while the 88 kDa form decreases to undetectable levels by E16, (Lanier, 1999). Mena contains 4 domains of interest; the Ena/VASP homologue 1 (EVH1) domain, an EVH2 domain, a proline-rich domain, and an Arg/Leu/Glu-risch region. Expression of Mena has been observed in the myoepithelia of breast, bronchial glands, parotid, and sweat glands. Mena has also been observed in the epithelium of mouse mucosal tissues: colon-rectum, endometrium, pancreas acinar ductal, placenta vascular media, and prostate fibromuscular stroma. Most breast cancer cell lines, along with lesions from primary breast tumor, demonstrate overexpression of Mena.

Immunogen

Histidine-tagged recombinant protein corresponding to human Mena and an additional fragment of Mena +, comprising only the + exon

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in rat hippocampus tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Mena in 10 µg of rat hippocampus tissue lysate.

Specificity

The antibody recognizes Mena and Mena alternate form (+ exon).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 80 kDa observed MW. In brain tissues, a 140 kDa MW band is also expected to be observed.